NGS Fragment Analyzer™ Fluorescent System Software Features.

Global Configuration Settings. For data files being processed for the first time, the Global Configuration Settings Box opens allowing the user to set the parameters for the analysis. Setting for picking peaks, markers and the ladder calibration are adjustable. Other criteria for calculating quantity and size of fragments can be set when the file opens, and set after the data is initially processed. Advanced settings allow the user to specify marker concentration, dilution factor and calculation mode (RNA, DNA, gDNA, NGS and Mutation Analysis)

Gel view. View all 96 capillaries at the same time. After normalization of all lanes to the upper/lower marker fragments, all of the samples can be compared simultaneously. Each electropherogram separation can be viewed separately, in this view, by moving through the gel view image or clicking through the 96 well plate image. Fragment size can be easily determined using the pull down ruler, showing fragment size on both the electropherogram and on the gel view. A table, which can be exported, shows both individual peak sizes and concentrations of the selected separation.

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Sample Overlay view. For a close up view of any sample, the sample overlay view is used. Gel order can be arranged as needed and the gel view image is right clickable for easy pasting into another electronic media. This view shows both a gel view image and the selected electropherograms. Further editing capabilities include: a sizing ruler; fragment color and background selection, copy/paste function and annotation.

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Flagging Feature. This feature was designed to reduce back end analysis time. Analytical parameters entered into the 'Flag' criteria windows can be saved and recalled for quick review of sample plates. Combining Boolean logic and limitation for bp units range or concentration allows for maximum flexibility. Results are provided in a table represented as a '1' or a '0' showing if the criteria were met.

Smear Analysis. NGS fragments, RNA and genomic DNA can be quickly analyzed after separation to size and quantify smears of nucleic acids. Smear concentration can be calculated several ways, either by the software or through user-defined smear boundaries. The software calculates the concentration in both ng/uL, nmole/L, Average Size and %CV. Sample information can be exported to Excel©.

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Report Generation. Data can be downloaded as a pdf or xls file extension for all or part of the samples. The report contents can be tailored to output specific and critical information and easily modified to include more or less information.

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