I want to. . .

Automate Throughput	Increase Throughput
Decrease Labor Prep Time	Lower Run Cost
Improve Quantity/Quality Assessment	Stop Using Manual Slab Gels
Improve Resolution

I want to Improve Quantity/Quality Assessment

Quality and quantity analysis of RNA is common. Accurate assessment of both quality and quantity is critical for many of the post-extraction applications such as microarray analysis, real time PCR, cDNA synthesis, gene expression studies, etc.

If researchers want to use analytical instruments for doing quantity and quality assessments, they have two choices: microfluidic or capillary-based.

The NGS Fragment Analyzer™ demonstrates both better sizing and quantification accuracy/reproducibility than any other analytical method (see Table). This means that results can be appraised with confidence for one sample, or 12 samples and from one run to the next.

	NGS Fragment Analyzer™ mRNA Kit-Pico Assay
Quantitation Precision	±20% RSD
Quantitation Accuracy (ladder as sample)	±30% Rel. Error
Sizing Precision (ladder as sample)	±10% Rel. Error
Sizing Accuracy	±20% Rel. Error
Limit of Detection	250 pg/µL
Qualitative Range (per smear)	500 to 15000 pg/µL
Quantitative Range	500 to 10000 pg/µL
Sample Amount Required	2µL
Number of Samples per Run	11 samples/plate + 1 wells RNA ladder
Analysis Time	30 minutes CE separation + 15 minutes conditioning

The following factors allow the application of NGS Fragment Analyzer™ for improving quantity and quality analysis of RNA:

1. A specifically formulated QC-performance test Kit for RNA analysis.
2. Superior instrument design - coupling a sensitive detector with a high quality optics system.
3. Superior capillary design - separation over 80cm capillary versus <1 cm micro channel.