I want to...

Stop Using Manual Slab Gels	Decrease Labor Prep Time
Improve Quantity/Quality Assessment	Automate Throughput

I want to Improve Quantity/Quality Assessment

Accurately assessing both the quality and quantity of plasmid DNA can be challenging. Until now, assessing quality (size) of plasmid DNA was typically done with agarose gel electrophoresis. Agarose gel, though easy to do, is inherently fraught with inconsistencies that can result from gel preparation variations and sample loading.

Also, ‘smiling effects’ caused by improper and uncontrolled electrophoresis conditions can lead to incorrect size assessment.Quantity analysis is equally tricky because UV and fluorometric methods look at all nucleic acids in the sample - not just the fragments of interest. Sometimes agarose gel is used for quantification. However, agarose can only provide 'ballpark' assessments, being subject to a myriad of experimental conditions.

The AdvanCE™ FS improve quality and quantity analysis of large DNA fragments. For starters, the system was designed to handle large DNA fragments. Currently two gel kits are available to separate large DNA fragments (DNF-920 and DNF-930).

By precisely controlling the electrophoresis conditions and separating the DNA through QC-performance tested gels and buffers, high separation resolution is achieved which results in accurate sizing (quality assessment) and quantification. Fragments are objectively quantified using analytical software especially designed and validated for this purpose. Gone are the days of ‘ballparking’ quantity.

The following factors allow the AdvanCE™ FS to improve quality and quantity assessments of large DNA fragments:

1. Superior resolution of large fragments.
2. Precise control of electrophoresis conditions and utilization of marker fragments.
3. PROSize™ analytical software.