I want to...

Automate Throughput	Decrease Labor Prep Time
Reduce Input gDNA	Stop Using Manual Slab Gels
Analzye Larger Fragments	Eliminate Clean Up Step
Increase Throughput	Reduce Time To Results
Eliminate Labeled Primers

I want to Eliminate Labeled Primers

Fluorescent labeled primers are more expensive than unlabelled primers. This fact dramatically increases the cost per unit to use fluorescent labeled primers in a PCR amplification. Another disadvantage of labeled primers is their short half-life, some being as short as 6 months. Other labeled primers are highly light-sensitive and require special handling procedures.

Labeled primers are NOT needed on the AdvanCE™ FS Nucleic Acids Analyzer.

Eliminating the need for fluorescent-labeled primers has many advantages, including: reduced primer costs; and the ability to move directly from primer development to primer usage without amplification inconsistencies. Eliminating the use of labeled primers can slice primer costs by 5-fold or more, depending on the label and level of purification needed after primer synthesis.

The following design features of the AdvanCE™ FS Nucleic Acids Analyzer allow the elimination of labeled primers:

1. Use of nondenaturing gels and a sensitive intercalating fluorescent dye.
2. A high wattage (1,000mW) LED and a sensitive CCD detector.
3. Low signal to noise ratio, typically at 3:1.