I want to...

Automate Throughput	Decrease Labor Prep Time
Reduce Input gDNA	Stop Using Manual Slab Gels
Analzye Larger Fragments	Eliminate Clean Up Step
Increase Throughput	Reduce Time To Results
Eliminate Labeled Primers

I want to Increase Throughput

Throughput increases can be gained by processing more samples per day and by decreasing sample handling/preparation time. Using the traditional method for reverse genetics TILLiNG, it is difficult to make changes to either of these.

With the AdvanCE™ FS Nucleic Acids Analyzer, more samples can be processed per day by potentially increasing pooling ratios. At the heart of this lies detection sensitivity. In mutation detection, higher pooling ratios allow more samples to be screened per experiment thus increasing throughput and efficiency.

Currently organisms are typically pooled up to 16 alleles (i.e. 8 diploid organisms, 4 tetraploids, 2 or 3 hexaplooids, etc). Work on the AdvanCE™ FS Nucleic Acids Analyzer has shown that higher pooling ratios are possible.

More samples can be processed per day with less hands-on time on the AdvanCE™ FS Nucleic Acids Analyzer, by eliminating gel plate preparations, shortening electrophoresis time and by decreasing sample handling. These time gains allow at least twice as many samples to be analyzed per day. Utilizing a batch processing model for the enzyme digestion and sample processing, additional organisms can be analyzed due to more efficient sample handling.

The following factors help increase throughput on the AdvanCE™ FS Nucleic Acids Analyzer:

1. Streamlined process that utilizes only 3 pipeting steps prior to electrophoresis
2. Fast electrophoresis times
3. The potential for higher pooling ratios