I want to...

Automate Throughput	Decrease Labor Prep Time
Reduce Input gDNA	Stop Using Manual Slab Gels
Analzye Larger Fragments	Eliminate Clean Up Step
Increase Throughput	Reduce Time To Results
Eliminate Labeled Primers

I want to Reduce Time to Results

Due to all the steps required using the traditional method for reverse genetics TILLinG, a single experiment from start (PCR) to finish (results generation) can take 9+ hours of total time with over 2 hours of hands-on time. In other words, the lab must dedicate a full day of human labor to finish one plate of samples.

But mutation detection is supposed to be a a screening tool to shift through the thousands or ten's of thousands of mutated organisms to determine which, if any, have a mutation in a specific gene. Excessive, intense labor works against the basic premise of screening.

So, the faster that screening can be accomplished, the sooner the mutated organisms can be studied and mutation effects documented.

The AdvanCE™ FS Nucleic Acids Analyzer doubles screening throughput by reducing the per plate analysis time down to 4 hours while at the same time decreasing the hands on time to under ¾ hour. Time reductions are gained by not having to manually prepare gels and reagents, and via fast electrophoresis times.

Also, gel formulas and instrument design allow fragments to be separated with high resolution and speed. In a typical electrophoresis run, DNA is simultaneously injected into and separated through 96 individual capillaries in as short as 30 minutes; run times can be increased to 60 minutes depending on the separation requirements (i.e. resolution needs).

The following design features help reduce time to results for mutation detection on the AdvanCE™ FS Nucleic Acids Analyzer:

1. No glass plate assembly/disassembly or gel preparation requirements.
2. Fast electrophoresis times - 30 minutes typical.
3. Only 3 pipeting steps post heteroduplex formation are required to prepare samples for electrophoresis.